| Product Name | Collagen IV antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Collagen IVCollagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. |
| Immunogen | Full length native protein (extracted and purified from tumor tissues) (Mouse). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PRIA |
| ELISA: 1/200. IF: 1/80. Use on frozen mouse tissues (skin, liver). IHC-P: 1/500. RIA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Synonyms | 120070 FLJ22259 1285 P02462 1284 1286 120130 P08572 OPLL COL4A4 Collagen IV COL4A2 CA44 120131 Q01955 COL6A1 COL4A3 1291 120090 1282 COL4A1 P12109 P53420 120220 arresten |
|---|---|
| Target | Collagen IV |
| Tissue Specificity | Recognises Mouse Collagen Type IV (100% by RIA). Does not react with Mouse Collagen Types I or III, Human Collagen Type IV or V, Mouse Fibronectin or Mouse Laminin ( |
| Cellular Localization | Secreted (Basement membrane). |
| Application Notes | ELISA: 1/200. IF: 1/80. Use on frozen mouse tissues (skin, liver). IHC-P: 1/500. RIA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
