Application
HEK293T cells transfected with either RC203023 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CORO1B antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CORO1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CORO1B.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CORO1B monoclonal antibody.Application
Anti-CORO1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CORO1B| Product Name | CORO1B antibody [3D10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3D10] to CORO1BRegulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction. |
| Immunogen | Full length human recombinant protein of human CORO1B (NP_065174) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3D10 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CORONIN-2, CORONIN2, CORO1B, DKFZP762I166, CORONIN 2 |
|---|---|
| Target | CORO1B |
| Tissue Specificity | is specific for human CORO1B (NP_065174). |
| Cellular Localization | Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers. |
Application
HEK293T cells transfected with either RC203023 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CORO1B antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CORO1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CORO1B.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CORO1B monoclonal antibody.
Application
Anti-CORO1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CORO1B| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
