Application
IHC Image using - Detection of COX2 by IHC in Human Colon CarcinomaApplication
Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody.| Product Name | COX2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Polyclonal Antibody to COX2This antibody reacts with a 70 kD protein. COX2 (Cyclooxygenase-2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters and cytokines that induce its expression. COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged. This antibody cross reacts with mouse and rat. |
| Immunogen | Recombinant protein corresponding to c-terminus of rat COX2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 29527, COX-2, Cox2, Ptgs2, COX 2 |
|---|---|
| Target | COX2 |
| Cellular Localization | Cytoplasmic, membrane |
Application
IHC Image using - Detection of COX2 by IHC in Human Colon Carcinoma
Application
Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody.| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | This antibody contains less than 0.1 % sodium azid. |
