Application
IHC-P analysis of colon carcinoma using COX2 antibody [4H12].| Product Name | COX2 antibody [4H12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4H12] to COX2 / Cyclooxygenase 2COX2 is a mitogen-inducible form of Cyclooxygenase, which is expressed in response to various inflammatory stimuli. COX2 staining increases in the more differentiated, suprabasal keratinocytes of normal skin. COX2 is expressed in epithelial and interstitial cells of adenomatous polyps and Colon adenocarcinomas. |
| Immunogen | Prokaryotic recombinant protein corresponding to amino acids 30 to 163 of the N-terminus of the human COX-2 molecule. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4H12 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| IHC-P: Use at a dilution of 1/25 - 1/50. Perform heat mediated antigen retrieval with 10mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. We suggest an incubation period of 60 minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | PHS-2, COX-2, hCox2, hCox-2, PGHS-2, PGG/HS, PGHS2, 5743, 600262, COX2, PTGS2, P35354, PHS2, PHS 2, COX 2, hCox 2, PGHS 2 |
|---|---|
| Target | COX2 |
| Cellular Localization | Microsomal membrane |
Application
IHC-P analysis of colon carcinoma using COX2 antibody [4H12].| Application Notes | IHC-P: Use at a dilution of 1/25 - 1/50. Perform heat mediated antigen retrieval with 10mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. We suggest an incubation period of 60 minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Tissue Culture Supernatant containing 0.05% sodium azide |
