Application
WB analysis of indicated lysates using COX4 antibody at a dilution of 1:5,000.Application
WB analysis of mouse skeletal muscle and Jurkat lysates using COX4 antibody at a dilution of 1:1,000.Application
FACS analysis of K562 cells using COX4 antibody at a dilution of 1:100 (red) or an isotype control (blue).Application
ICC/IF analysis of HeLa cells using COX4 antibody at a dilution of 1:150.Application
IP analysis of HeLa lysates using control IgG and COX4 antibody. The precipitates were detected by the same COX4 antibody.| Product Name | COX4 antibody [4D11-B3-E8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4D11-B3-E8] to COX4Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain.It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane.The complex consists of 13 mitochondrial- and nuclear-encoded subunits.The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities.The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex.This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme.It is located at the 3´ of the NOC4 (neighbor of COX4) gene in a head-to-head orientation,and shares a promoter with it.[provided by RefSeq,Jul 2008] |
| Immunogen | Purified recombinant human COX4 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4D11-B3-E8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIPWB |
| Species Reactivity | HumanMouseMonkeyRatGoatHamster |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | COXIV , COX4 , COX4I1 , COX4-1 , cytochrome c oxidase subunit IV isoform 1 |
|---|---|
| Target | COX IV |
| Molecular Weight(MW) | 17kDa (note) |
| Cellular Localization | Mitochondrion inner membrane |
Application
WB analysis of indicated lysates using COX4 antibody at a dilution of 1:5,000.
Application
WB analysis of mouse skeletal muscle and Jurkat lysates using COX4 antibody at a dilution of 1:1,000.
Application
FACS analysis of K562 cells using COX4 antibody at a dilution of 1:100 (red) or an isotype control (blue).
Application
ICC/IF analysis of HeLa cells using COX4 antibody at a dilution of 1:150.
Application
IP analysis of HeLa lysates using control IgG and COX4 antibody. The precipitates were detected by the same COX4 antibody.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol. |
