Application
Western blot analysis using COX4I1 mouse mAb against HEK293 (1), A549 (2) and PC12 (3) cell lysateApplication
Immunofluorescence analysis of PANC-1 cells using COX4I1 mouse mAb ( green). Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidinApplication
Flow cytometric analysis of K562 cells using COX4I1 mouse mAb ( blue) and negative control (red)| Product Name | COX4 antibody [6B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6B3] to COX4I1Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human COX4I1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6B3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000. | |
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Gene Synonyms | COX4-1 , COX4 , COXIV |
|---|---|
| Target | COX4 |
| Molecular Weight(MW) | 19 kDa (note) |
Application
Western blot analysis using COX4I1 mouse mAb against HEK293 (1), A549 (2) and PC12 (3) cell lysate
Application
Immunofluorescence analysis of PANC-1 cells using COX4I1 mouse mAb ( green). Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin
Application
Flow cytometric analysis of K562 cells using COX4I1 mouse mAb ( blue) and negative control (red)| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
