Application
COX4 antibody [GT854] detects COX4I1 protein by western blot analysis. A. 50 µg rat muscle lysate/extract 15% SDS-PAGE COX4 antibody [GT854] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
COX4 antibody detects COX4I1 protein by western blot analysis. A. 50 µg mouse heart lysate/extract 12% SDS-PAGE COX4 antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
COX4 antibody [GT854] detects COX4I1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE COX4 antibody [GT854] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
COX4 antibody [GT854] detects COX4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: COX4 protein stained by COX4 antibody [GT854] diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | COX4 antibody [GT854] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT854] to COX4 (cytochrome c oxidase subunit IV isoform 1)Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | GT854 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse,Rat |
| Predicted Reactivity | Rhesus Monkey, Chimpanzee, BovineRhesus Monkey (92%), Chimpanzee (100%), Bovine (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 20 kDa (note) (2) |
|---|---|
| Cellular Localization | Mitochondrion inner membrane |
Application
COX4 antibody [GT854] detects COX4I1 protein by western blot analysis. A. 50 µg rat muscle lysate/extract 15% SDS-PAGE COX4 antibody [GT854] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
COX4 antibody detects COX4I1 protein by western blot analysis. A. 50 µg mouse heart lysate/extract 12% SDS-PAGE COX4 antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
COX4 antibody [GT854] detects COX4I1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE COX4 antibody [GT854] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
COX4 antibody [GT854] detects COX4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: COX4 protein stained by COX4 antibody [GT854] diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
