An ELISA plate is coated with 50 μL of a C-peptide at concentration ranged from 0.9 μg/mL to 0.001 μg/mL. The coated peptide is detected with anti C-peptide antibody at 0.5 μg/mL.
Mouse Monoclonal antibody [GT1455] to C-peptide (insulin)After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq]
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Insulin. The exact sequence is proprietary.
Key Feature
Clonality
Monoclonal
Isotype
IgG2
Clone Number
GT1455
Host Species
Mouse
Tested Applications
ELISA
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human
Predicted Reactivity
ChimpanzeeChimpanzee (100%)
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
INS , insulin , IRDN , IDDM2 , MODY10 , ILPR
Target
C-peptide
Molecular Weight(MW)
12 kDa (note) (4)
Cellular Localization
Secreted
Application
Application
An ELISA plate is coated with 50 μL of a C-peptide at concentration ranged from 0.9 μg/mL to 0.001 μg/mL. The coated peptide is detected with anti C-peptide antibody at 0.5 μg/mL.
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.