Application
Antigen retrival was done with Tris-EDTA, pH 9.0 in pressure cooker for 20 minutes. Strong and specific staining for islet cells were observed.Application
Peptide was coated at 0.5 µp per well: Serial dilution of the antibody starting with 2 µg/well was done to check for the affinity. 0.5 µg of coated antigen can be sensitively detected by the anti-human C-peptide antibody, used at 100 pg/well concentration| Product Name | C-Peptide antibody [HCP-B2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [HCP-B2] to C-PeptideC Peptide is part of the molecule of Proinsulin, the insulin precursor molecule, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. The ratio of C-peptide and Proinsulin in human serum is also very important in diagnosis and prognosis of various diseases like Polycystic Ovary Syndrome, Ovarian carcinoma, etc. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HCP-B2 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC |
| The antibody can be used for indirect ELISA and IHC. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C peptide, Cpeptide |
|---|---|
| Target | C-Peptide |
| Tissue Specificity | This antibody is specific for C-peptide. |
Application
Antigen retrival was done with Tris-EDTA, pH 9.0 in pressure cooker for 20 minutes. Strong and specific staining for islet cells were observed.
Application
Peptide was coated at 0.5 µp per well: Serial dilution of the antibody starting with 2 µg/well was done to check for the affinity. 0.5 µg of coated antigen can be sensitively detected by the anti-human C-peptide antibody, used at 100 pg/well concentration| Application Notes | The antibody can be used for indirect ELISA and IHC. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 0.05% (W/V) sodium azide |
