Application
Cell lysates of mouse eye (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP2 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | CRABP2 antibody [AT2E11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT2E11] to CRABP2The cellular retinoic acid-binding protein II (CRABP-II) is involved in the conversion of vitamin A into its intracellular active form retinoic acid, which regulate the genes responsible for lipid metabolism and adipocyte differentiation. CRABP2 gene is located on chromosome 1q21-23 and this region has been linked with related disorders such as familial combined hyperlipidemia (FCHL) and type 2 diabetes mellitus. |
| Immunogen | Recombinant human CRABP2 (1-138aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AT2E11 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| Western Blot: Use at 1:250 ~ 1000. Optimal working concentrations should be determined experimentally by the end user. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRABP-II, CRABP2, RBP6, CRABP II, CRABPII, Cellular retinoic acid binding protein 2, Cellular retinoic acid binding protein2, Cellular retinoic acid binding protein2 Cellular retinoic acid binding protein II, Cellular retinoic acid binding protein 2 Ce |
|---|---|
| Target | CRABP2 |
| Cellular Localization | Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. |
| Light Chain Type | kappa |
Application
Cell lysates of mouse eye (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP2 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:250 ~ 1000. Optimal working concentrations should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
