Application
Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of Cre-tagged protein expressing HeLa cells 7.5% SDS-PAGE Cre recombinase antibody [GT10212] dilution: 1:2000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis. A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins 12.5% SDS-PAGE Cre recombinase antibody [GT10212] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Cre recombinase antibody immunoprecipitates Cre recombinase protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 μg of Cre recombinase antibody 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody diluted at 1 : 2000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.| Product Name | Cre recombinase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Cre recombinase |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT10212 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| WB:1:500~1:2000 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Cre antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
Application
Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg whole cell lysate/extract of Cre-tagged protein expressing HeLa cells 7.5% SDS-PAGE Cre recombinase antibody [GT10212] dilution: 1:2000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis. A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins 12.5% SDS-PAGE Cre recombinase antibody [GT10212] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Cre recombinase antibody immunoprecipitates Cre recombinase protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 μg of Cre recombinase antibody 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody diluted at 1 : 2000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.| Positive Control | Cre-tagged protein expressing HeLa , IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins |
|---|---|
| Application Notes | WB:1:500~1:2000 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
