Application
CREST antibody [C1C3] detects CREST protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.Application
CREST antibody [C1C3] detects CREST protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CREST protein stained by CREST antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
CREST antibody [C1C3] detects CREST protein by western blot analysis. U87-MG whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000Application
CREST antibody [C1C3] detects CREST protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.| Product Name | CREST antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREST (synovial sarcoma translocation gene on chromosome 18-like 1)Synovial sarcomas occur most frequently in the extremities around large joints. More than 90% of cases have a recurrent and specific chromosomal translocation, t(X;18)(p11.2;q11.2), in which the 5-prime end of the SS18 gene (MIM 600192) is fused in-frame to the 3-prime end of the SSX1 (MIM 312820), SSX2 (MIM 300192), or SSX4 (MIM 300326) gene. The SS18L1 gene is homologous to SS18.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CREST. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CREST antibody KIAA0693 antibody LP2261 antibody MGC26711 antibody MGC78386 antibody SS18L1 antibody SS18-like protein 1 antibody calcium-responsive transactivator antibody SYT homolog 1 antibody synovial sarcoma translocation gene on chromosome 1 |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (3) |
Application
CREST antibody [C1C3] detects CREST protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.
Application
CREST antibody [C1C3] detects CREST protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CREST protein stained by CREST antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
CREST antibody [C1C3] detects CREST protein by western blot analysis. U87-MG whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000
Application
CREST antibody [C1C3] detects CREST protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.| Positive Control | MCF-7 , Mouse brain , Rat brain , U87-MG whole cell extract , U87-MG NE nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
