Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000Application
Immunoprecipitation of CREST protein from MCF-7 whole cell extracts using 5 μg of CREST antibody [N1C2] . Western blot analysis was performed using CREST antibody [N1C2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CREST antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using CREST antibody at 1:500 dilution.Application
Sample (20 ug ) A: Hela whole cell lysate B: HeLa nucleus 10% SDS PAGE diluted at 1:1000| Product Name | CREST antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CREST (synovial sarcoma translocation gene on chromosome 18-like 1)Synovial sarcomas occur most frequently in the extremities around large joints. More than 90% of cases have a recurrent and specific chromosomal translocation, t(X;18)(p11.2;q11.2), in which the 5-prime end of the SS18 gene (MIM 600192) is fused in-frame to the 3-prime end of the SSX1 (MIM 312820), SSX2 (MIM 300192), or SSX4 (MIM 300326) gene. The SS18L1 gene is homologous to SS18.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CREST. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (81%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CREST antibody KIAA0693 antibody LP2261 antibody MGC26711 antibody MGC78386 antibody SS18L1 antibody SS18-like protein 1 antibody calcium-responsive transactivator antibody SYT homolog 1 antibody synovial sarcoma translocation gene on chromosome 1 |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (2) |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:1000
Application
Immunoprecipitation of CREST protein from MCF-7 whole cell extracts using 5 μg of CREST antibody [N1C2] . Western blot analysis was performed using CREST antibody [N1C2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CREST antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using CREST antibody at 1:500 dilution.
Application
Sample (20 ug ) A: Hela whole cell lysate B: HeLa nucleus 10% SDS PAGE diluted at 1:1000| Positive Control | HeLa , HeLa nucleus , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
