Application
WB analysis of LNCAP, A431, MCF7 and HT1080 lysates using CRK antibody at a dilution of 1:1,000.| Product Name | CRK antibody [3H7-E5-H8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3H7-E5-H8] to CRKThis gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. |
| Immunogen | Purified recombinant human CRK protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3H7-E5-H8 |
| Host Species | Mouse |
| Tested Applications | WB |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CRKII , p38 , v-crk sarcoma virus CT10 oncogene homolog (avian) , CRK |
|---|---|
| Target | CrkII |
| Molecular Weight(MW) | 34kDa (note) |
| Cellular Localization | Cytoplasm, Cell membrane |
Application
WB analysis of LNCAP, A431, MCF7 and HT1080 lysates using CRK antibody at a dilution of 1:1,000.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
