Application
Sample: EL-4 mouse cell line. diluted at 1:250.Application
Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk ( green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).| Product Name | CSK antibody [CSK-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CSK - 04] to CSKC-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C-terminal tyrosine of Src family kinases thereby down-regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft-associated protein PAG (Csk-binding protein). |
| Immunogen | Recombinant fragment: VSDFGLTKEAS STQDTGKLPV KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK NCWHLDAAMR PSFLQLREQL EHIKTHELHL, corresponding to amino acids 330-450 of Human CSK. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CSK-04 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| IP: Use at an assay dependent dilution. The immunosorbent ( GTX14996 covalently bound to beads) immunoprecipitates CSK from cell lysates. WB: Use at a dilution of 1/250 - 1/300. Predicted molecular weight: 54 kDa. Weak non-specific band of approx. 60 kDa is detected in human cell lines in lower antibody dilutions. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | MGC117393, 1445, CSK, 124095, P41240 |
|---|---|
| Target | CSK |
| Cellular Localization | Nuclear |
Application
Sample: EL-4 mouse cell line. diluted at 1:250.
Application
Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk ( green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).| Application Notes | IP: Use at an assay dependent dilution. The immunosorbent ( GTX14996 covalently bound to beads) immunoprecipitates CSK from cell lysates. WB: Use at a dilution of 1/250 - 1/300. Predicted molecular weight: 54 kDa. Weak non-specific band of approx. 60 kDa is detected in human cell lines in lower antibody dilutions. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8ºC. Do not freeze. Do not use after expiration date stamped on vial label. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
