Application
Immunofluorescent staining of A549 cells using anti-CTAG1B mouse monoclonal antibody .Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.Application
Anti-CTAG1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CTAG1BApplication
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTAG1B monoclonal antibody.| Product Name | CTAG1B antibody [3F10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3F10] to CTAG1BCancer/testis antigens, such as CTAG1B, are expressed in a variety of malignant tumors but soley in testis among normal adult tissues (Yoshida et al., 2006 [PubMed 16596224]).[supplied by OMIM] |
| Immunogen | Protein expressed in 293T cell transfected with human CTAG1B expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTAG, CTAG1A, LAGE2B, CTAG1B, CTAG1, ESO1, CT6.1, LAGE2, NYESO1 |
|---|---|
| Target | CTAG1B |
| Tissue Specificity | is specific for human CTAG1B. |
Application
Immunofluorescent staining of A549 cells using anti-CTAG1B mouse monoclonal antibody .
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.
Application
Anti-CTAG1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CTAG1B
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTAG1B monoclonal antibody.| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
