Application
Western blot analysis using CTCF mouse mAb against A31 (1), MCF-7 (2), Hela (3), HCT116 (4), Jurkat (5), NIH/3T3 (6), and Cos7 (7) cell lysate.| Product Name | CTCF antibody [1D11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1D11] to CTCFThis gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Immunogen | The immunogen corresponding to human CTCF expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D11 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: 1/10000. | |
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Target | CTCF |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) |
Application
Western blot analysis using CTCF mouse mAb against A31 (1), MCF-7 (2), Hela (3), HCT116 (4), Jurkat (5), NIH/3T3 (6), and Cos7 (7) cell lysate.| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
