Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CtIP antibody at 1:100 dilution.| Product Name | CtIP antibody [19E8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human CtIPCtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disruppted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation. |
| Immunogen | Human CtIP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 19E8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWBChIP |
| For WB: Use at a dilution of 1:500-1:1000 in 3% Milk/TBST overnight.ChIP,IP:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CtIP, Q99708, 5932, 604124, RBBP8, RIM |
|---|---|
| Target | CtIP |
| Molecular Weight(MW) | 125 kDa (note) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CtIP antibody at 1:100 dilution.| Application Notes | For WB: Use at a dilution of 1:500-1:1000 in 3% Milk/TBST overnight.ChIP,IP:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
