Application
Western Blot: CUGBP1 Antibody (3B1) - Detection of CUG-BP1 in several cell lysates.Application
Immunocytochemistry/Immunofluorescence: CUGBP1 Antibody (3B1) - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.| Product Name | CUG-BP antibody [3B1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3B1] to CUG - BP1Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3-UTR. The (CUG)n repeat region in Mt-PK mRNA is a binding site for CUG-BP/hNab50 in vivo, and triplet repeat expansion leads to sequestration of this hnRNP on mutant Mt-PK transcripts. |
| Immunogen | CUG-BP1 human nuclear RNA binding protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3B1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:500 (ECL). A 50 kDa band can be seen in a Western blot analysis, using HeLa cell lysate. For IF: Use at a dilution of 1:500. Nuclear staining can be seen through immunofluorescence. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CUGBP, NAB50, CUGBP1, hNab50, BRUNOL2, Q92879, CUG-BP, 10658, 601074, CUG BP |
|---|---|
| Target | CUG-BP |
| Tissue Specificity | This antibody is specific for human CUG-BP1. |
| Light Chain Type | kappa |
Application
Western Blot: CUGBP1 Antibody (3B1) - Detection of CUG-BP1 in several cell lysates.
Application
Immunocytochemistry/Immunofluorescence: CUGBP1 Antibody (3B1) - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.| Application Notes | For WB: Use at a dilution of 1:500 (ECL). A 50 kDa band can be seen in a Western blot analysis, using HeLa cell lysate. For IF: Use at a dilution of 1:500. Nuclear staining can be seen through immunofluorescence. Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20 to -80 degrees C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Ascitic fluid with sodium azide |
