Application
IHC-P analysis of human carcinoid tissue using IP10 antibody at a concentration of 1.0 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.Application
Sandwich To detect hIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody- which is biotinylated allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10.| Product Name | CXCL10 / IP10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IP10This gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant Human IP-10 (CXCL10) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PNeutWB |
| We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 µg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 1.0 µg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Human IP-10 (100.0 ng/ml), a concentration of 5.0 – 8.0 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | INP10 crg-2 C7 IFI10 IP-10 SCYB10 mob-1 chemokine (C-X-C motif) ligand 10 gIP-10 CXCL10 |
|---|---|
| Target | IP10 |
Application
IHC-P analysis of human carcinoid tissue using IP10 antibody at a concentration of 1.0 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
Application
Sandwich To detect hIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody- which is biotinylated allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10.| Application Notes | We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 µg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 1.0 µg/ml. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Human IP-10 (100.0 ng/ml), a concentration of 5.0 – 8.0 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2 |
