Application
Western blot analysis of Human IL-8 using anti-human IL-8 mAb 807. The antibody when used at concentration of 0.02-0.1 ug/mL will allow visualization of 100 ng/lane ofh uman IL-8.| Product Name | CXCL8 / IL8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [807] to IL8IL8, formerly called monocyte derived neutrophil chemotactic factor, belongs to the chemokine alpha family. Human endothelial IL8 is an 8.9 kDa protein containing 77 amino acid residues. IL8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils and promotes degranulation. |
| Immunogen | Purified recombinant human IL-8. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 807 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| ELISA: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 0.02 - 0.1 μg/ml. Predicted molecular weight: 12 kDa. Optimal dilutions/concentrations should be determined by the end user. Concentration of 0.02-0.1 ug/ml of GTX18672 will allow visualization of 100 ng/lane of human IL-8.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | Inteleukin8 Inteleukin 8 310C NAP1 T cell chemotactic factor CXC chemokine ligand 8 Monocyte derived neutrophil chemotactic factor CXCL8 Granulocyte chemotactic protein 1 Neutrophil activating factor CXC chemokine ligand8 MONAP GCP1 Granulocyt |
|---|---|
| Target | IL8 |
| Tissue Specificity | This antibody recognizes both, recombinant and native human IL-8. This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted). |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
Application
Western blot analysis of Human IL-8 using anti-human IL-8 mAb 807. The antibody when used at concentration of 0.02-0.1 ug/mL will allow visualization of 100 ng/lane ofh uman IL-8.| Application Notes | ELISA: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 0.02 - 0.1 μg/ml. Predicted molecular weight: 12 kDa. Optimal dilutions/concentrations should be determined by the end user. Concentration of 0.02-0.1 ug/ml of GTX18672 will allow visualization of 100 ng/lane of human IL-8.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: 0.01M PBS. pH 7.2 |
