Application
Western blot analysis of Human IL-8 using clone I8-60. Concentration of 0.02-0.1 ug/mL antibody will allow visualization of 100 ng/lane of human IL-8.| Product Name | CXCL8 / IL8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [I8-60] to IL8IL8, formerly called monocyte derived neutrophil chemotactic factor, belongs to the chemokine alpha family. Human endothelial IL8 is an 8.9 kDa protein containing 77 amino acid residues. IL8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils and promotes degranulation. |
| Immunogen | Purified recombinant (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | I8-60 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| For ELISA: Use at an assay dependent dilution. Can be used as a detection antibody in an ELISA pair with GTX18649 (capture). For Neut: Use at a concentration of 5 μg/ml with 10 ng/ml IL8 for 98.4% inhibition of chemotactic activity. For WB: Use at a concentration of 0.02 - 0.1 μg/ml. Predicted molecular weight: 11 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | Inteleukin8 Inteleukin 8 310C NAP1 T cell chemotactic factor CXC chemokine ligand 8 Monocyte derived neutrophil chemotactic factor CXCL8 Granulocyte chemotactic protein 1 Neutrophil activating factor CXC chemokine ligand8 MONAP GCP1 Granulocyt |
|---|---|
| Target | IL8 |
| Tissue Specificity | This antibody recognises both recombinant and native IL8. It does not cross react with Monocyte Chemotactic Activating Factor (MCAF)or Regulated on Activation, Normal T-cell Expressed, and Secreted (RANTES). |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
Application
Western blot analysis of Human IL-8 using clone I8-60. Concentration of 0.02-0.1 ug/mL antibody will allow visualization of 100 ng/lane of human IL-8.| Application Notes | For ELISA: Use at an assay dependent dilution. Can be used as a detection antibody in an ELISA pair with GTX18649 (capture). For Neut: Use at a concentration of 5 μg/ml with 10 ng/ml IL8 for 98.4% inhibition of chemotactic activity. For WB: Use at a concentration of 0.02 - 0.1 μg/ml. Predicted molecular weight: 11 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.0, no preservative added |
