Application
Western blot analysis of Human IL-8 using clone I8-S2. The antibody, when used at concentration of 0.02-0.1 ug/mL antibody will allow visualization of 100 ng/lane of recombinant human IL-8.| Product Name | CXCL8 / IL8 antibody [I8-S2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [I8-S2] to IL8IL8, formerly called monocyte derived neutrophil chemotactic factor, belongs to the chemokine alpha family. Human endothelial IL8 is an 8.9 kDa protein containing 77 amino acid residues. IL8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils and promotes degranulation. |
| Immunogen | Purified recombinant IL8 (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | I8-S2 |
| Host Species | Mouse |
| Tested Applications | ELISA,Neut,WB |
| For ELISA: Use at an assay dependent dilution. Can be used as a capture antibody in an ELISA pair with GTX18656 (detection). For Neut: Use at an assay dependent dilution. For WB: Use at a concentration of 0.02 - 0.1 μg/ml. Predicted molecular weight: 11 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | Inteleukin8, Inteleukin 8, 310C, NAP1, T cell chemotactic factor, CXC chemokine ligand 8, Monocyte derived neutrophil chemotactic factor, CXCL8, Granulocyte chemotactic protein 1, Neutrophil activating factor, CXC chemokine ligand8, MONAP, GCP1, Granulocyt |
|---|---|
| Target | IL8 |
| Tissue Specificity | This antibody recognises both recombinant and native IL8. This antibody does not cross react with Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted). |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
Application
Western blot analysis of Human IL-8 using clone I8-S2. The antibody, when used at concentration of 0.02-0.1 ug/mL antibody will allow visualization of 100 ng/lane of recombinant human IL-8.| Application Notes | For ELISA: Use at an assay dependent dilution. Can be used as a capture antibody in an ELISA pair with GTX18656 (detection). For Neut: Use at an assay dependent dilution. For WB: Use at a concentration of 0.02 - 0.1 μg/ml. Predicted molecular weight: 11 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.0, no preservative added |
