Application
Anti-CYB5R3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CYB5R3Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.| Product Name | CYB5R3 antibody [1E3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1E3] to CYB5R3Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. |
| Immunogen | Full length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E3 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,WB |
| ICC/IF 1:100, | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYB5R3, B5R, DIA1 |
|---|---|
| Target | CYB5R3 |
| Tissue Specificity | is specific for human CYB5R3 (NP_000389). |
| Cellular Localization | Produces the soluble form found in erythrocytes. |
Application
Anti-CYB5R3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CYB5R3
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.| Application Notes | ICC/IF 1:100, |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
