Application
HEK293T cells transfected with either RC201592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYB5R3 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Flow cytometric Analysis of Jurkat cells, using anti-CYB5R3 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYB5R3 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody.| Product Name | CYB5R3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2A10] to CYB5R3Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. |
| Immunogen | Full length human recombinant protein of human CYB5R3 (NP_000389) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2A10 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-PWB |
| IHC 1:150 : |
|
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYB5R3 B5R DIA1 |
|---|---|
| Target | CYB5R3 |
| Tissue Specificity | is specific for human CYB5R3 (NP_000389). |
| Cellular Localization | Produces the soluble form found in erythrocytes. |
Application
HEK293T cells transfected with either RC201592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CYB5R3 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Flow cytometric Analysis of Jurkat cells, using anti-CYB5R3 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYB5R3 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CYB5R3 mouse monoclonal antibody.| Application Notes | IHC 1:150 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
