Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG C: SK-N-SH 10% SDS PAGE diluted at 1:1000Application
Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Mouse and Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000.| Product Name | Cyclin A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CCNA2 (cyclin A2)The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rabbit, Rhesus Monkey, BovinePig (94%), Rabbit (92%), Rhesus Monkey (98%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCN1 antibody CCNA antibody CCNA2 antibody cyclin-A antibody cyclin-A2 antibody cyclin A2 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG C: SK-N-SH 10% SDS PAGE diluted at 1:1000
Application
Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Mouse and Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000.| Positive Control | NT2D1 , U87-MG , SK-N-SH , Mouse brain , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
