Application
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution.Application
Cyclin A2 antibody detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 protein stained by Cyclin A2 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.Application
Cyclin A2 antibody detects CCNA2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg A375 whole cell lysate/extract 10% SDS-PAGE Cyclin A2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cyclin A2 antibody detects CCNA2 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10% SDS-PAGE Cyclin A2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cyclin A2 antibody detects CCNA2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 10% SDS-PAGE Cyclin A2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Product Name | Cyclin A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin A2 (cyclin A2)The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rabbit, Rhesus Monkey, BovinePig (89%), Rabbit (83%), Rhesus Monkey (98%), Bovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCN1 antibody CCNA antibody CCNA2 antibody cyclin-A antibody cyclin-A2 antibody cyclin A2 antibody |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution.
Application
Cyclin A2 antibody detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin A2 protein stained by Cyclin A2 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Cyclin A2 antibody detects CCNA2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg A375 whole cell lysate/extract 10% SDS-PAGE Cyclin A2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cyclin A2 antibody detects CCNA2 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10% SDS-PAGE Cyclin A2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cyclin A2 antibody detects CCNA2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract 10% SDS-PAGE Cyclin A2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Positive Control | 293T , HeLa , A375 , Neuro2A , SK-N-SH , IMR32 , SK-N-AS , PC-12 , HeLa(100 µg/ml Bleomycin treatment for 24 hr) , *PC3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
