Application
Western blot analysis of extracts from MCF7 tissue using Cyclin D1 (Phospho-Thr286) antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Cyclin D1 (phospho Thr286) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin D1 (phospho Thr286)The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of threonine 286 (A-C-T(p)-P-T) derived from Human Cyclin D1. |
| Modification | p-Thr286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCND1 BCL1 D11S287E U21B31 PRAD1 cyclin D1 |
|---|---|
| Target | Cyclin D1 (phospho Thr286) |
| Tissue Specificity | The antibody detects endogenous level of Cyclin D1 only when phosphorylated at threonine 286. |
Application
Western blot analysis of extracts from MCF7 tissue using Cyclin D1 (Phospho-Thr286) antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
