Application
Cyclin D1 antibody [N2C3] detects Cyclin D1 protein at nucleus and cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cyclin D1 antibody [N2C3] dilution: 1:500.Application
Cyclin D1 antibody [N2C3] detects Cyclin D1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Cyclin D1 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cyclin D1 (cyclin D1)The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cyclin D1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Dog, Chicken, Rhesus Monkey, BovineRat (95%), Xenopus laevis (80%), Dog (93%), Chicken (88%), Rhesus Monkey (99%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCL1 antibody D11S287E antibody PRAD1 antibody U21B31 antibody CCND1 antibody B-cell CLL/lymphoma 1 antibody PRAD1 oncogene antibody G1/S-specific cyclin D1 antibody B-cell lymphoma 1 protein antibody BCL-1 oncogene antibody G1/S-specific cyclin- |
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| Molecular Weight(MW) | 34 kDa (note) |
Application
Cyclin D1 antibody [N2C3] detects Cyclin D1 protein at nucleus and cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Cyclin D1 antibody [N2C3] dilution: 1:500.
Application
Cyclin D1 antibody [N2C3] detects Cyclin D1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
