Application
WB analysis of Jurkat, K562 and A431 lysates using Cyclin H antibody at a dilution of 1:1,000.Application
IP analysis of HeLa lysates using control IgG and Cyclin H antibody. The precipitates were detected by the same Cyclin H antibody.| Product Name | Cyclin H antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4E11-G2-D7] to Cyclin HThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[ |
| Immunogen | Purified recombinant human CCNH(N-term) protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4E11-G2-D7 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cyclin H CAK p34 CCNH p37 |
|---|---|
| Target | Cyclin H |
| Molecular Weight(MW) | 38kDa (note) |
| Cellular Localization | Nucleus. |
Application
WB analysis of Jurkat, K562 and A431 lysates using Cyclin H antibody at a dilution of 1:1,000.
Application
IP analysis of HeLa lysates using control IgG and Cyclin H antibody. The precipitates were detected by the same Cyclin H antibody.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50% glycerol |
