Application
Cell lysates of HepG2(30ug) and HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection| Product Name | Cyclophilin B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [ k2E2] to Cyclophilin BCyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Cyclophilin B is necessary for the prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin. |
| Immunogen | Recombinant human Cyclophilin B (26-216aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | k2E2 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 26216aa CYPS1: CyclophilinB 26- 216aa MGC14109 AA408962 Cphn 2 PPIase MGC2224 Peptidyl prolyl cis trans isomerase B precursor CYP-S1: Cyclophilin-B CyP20b CYP S1 26 216a AA553318 CyP 20b peptidyl prolyl cis trans isomerase B S cyclophilin. |
|---|---|
| Target | Cyclophilin B |
| Cellular Localization | Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Light Chain Type | kappa |
Application
Cell lysates of HepG2(30ug) and HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection| Application Notes | Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
