Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytochrome P450 1B1 antibody at 1:200 dilution.Application
CYP1B1 antibody detects CYP1B1 protein by western blot analysis. A. 50 µg mouse lung lysate/extract 7.5% SDS-PAGE CYP1B1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CYP1B1 antibody detects CYP1B1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract 7.5% SDS-PAGE CYP1B1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CYP1B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYP1B1 (cytochrome P450, family 1, subfamily B, polypeptide 1)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP1B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Rhesus Monkey, BovineRat (84%), Dog (82%), Rhesus Monkey (94%), Bovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CP1B antibody GLC3A antibody P4501B1 antibody CYP1B1 antibody aryl hydrocarbon hydroxylase antibody microsomal monooxygenase antibody cytochrome P450 1B1 antibody xenobiotic monooxygenase antibody cytochrome P450 subfamily I (dioxin-inducible) po |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane , Microsome membrane |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytochrome P450 1B1 antibody at 1:200 dilution.
Application
CYP1B1 antibody detects CYP1B1 protein by western blot analysis. A. 50 µg mouse lung lysate/extract 7.5% SDS-PAGE CYP1B1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CYP1B1 antibody detects CYP1B1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract 7.5% SDS-PAGE CYP1B1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , mouse lung |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
