Application
Western blot analysis of Cytochrome P450 3A7 in 25 ug of HepG2, HeLa and HT29 cell lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with Cytochrome P450 3A7 antibody [F19 P2 H2] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.Application
Flow cytometry analysis of Cytochrome P450 3A7 in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Cytochrome P450 3A7 antibody [F19 P2 H2] at a dilution of 1:80 for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated in a proper secondary antibody, and re-suspended in PBS for FACS analysis.Application
Immunohistochemistry analysis of Cytochrome P450 3A7 in paraffin-embedded human kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Cytochrome P450 3A7 antibody [F19 P2 H2] diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.| Product Name | CYP3A7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [F19 P2 H2] to CYP3A7This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. The enzyme also metabolizes some drugs such as aflatoxin B1. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Transcript variants have been described, but it is not known whether these transcripts are normally produced. This gene undergoes readthrough transcription with the downstream CYP3AP1 pseudogene, resulting in an isoform with a novel C-terminus, as represented in GeneID:100861540. [provided by RefSeq, Feb 2012] |
| Immunogen | Synthetic peptide corresponding to residues E S R D E T V S G A, conjugated to ovalbumin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | F19 P2 H2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHCIHC-PWB |
| IHC-P: 1:100 - 1:500: |
|
| Species Reactivity | HumanBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | cytochrome P450 family 3 subfamily A polypeptide 7 P450-HFLA CP37 CYP3A7 CYPIIIA7 |
|---|---|
| Target | CYP3A7 |
Application
Western blot analysis of Cytochrome P450 3A7 in 25 ug of HepG2, HeLa and HT29 cell lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with Cytochrome P450 3A7 antibody [F19 P2 H2] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
Application
Flow cytometry analysis of Cytochrome P450 3A7 in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Cytochrome P450 3A7 antibody [F19 P2 H2] at a dilution of 1:80 for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated in a proper secondary antibody, and re-suspended in PBS for FACS analysis.
Application
Immunohistochemistry analysis of Cytochrome P450 3A7 in paraffin-embedded human kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Cytochrome P450 3A7 antibody [F19 P2 H2] diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.| Application Notes | IHC-P: 1:100 - 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Ascites containing 0.05 % sodium azide |
