Application
CYP24A1 antibody detects CYP24A1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CYP24A1 antibody dilution: 1:500.Application
Sample(30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CYP24A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYP24A1 (cytochrome P450, family 24, subfamily A, polypeptide 1)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP24A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, PigRat (87%), Pig (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CP24 antibody CYP24 antibody MGC126273 antibody MGC126274 antibody P450-CC24 antibody CYP24A1 antibody 24-OHase antibody cytochrome P450 subfamily XXIV (vitamin D 24-hydroxylase) antibody cytochrome P450-CC24 antibody vitamin D(3) 24-hydroxylase |
|---|---|
| Target | CYP24A1 |
| Molecular Weight(MW) | 59 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
CYP24A1 antibody detects CYP24A1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CYP24A1 antibody dilution: 1:500.
Application
Sample(30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
