Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using CYP27A1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CYP27A1 antibody detects CYP27A1 protein by western blot analysis. A. 50 µg rat liver lysate/extract 7.5% SDS-PAGE CYP27A1 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse Liver 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CYP27A1 antibody detects CYP27A1 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. CYP27A1 antibody diluted at 1:500.| Product Name | CYP27A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYP27A1 (cytochrome P450, family 27, subfamily A, polypeptide 1)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP27A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rabbit, Rhesus Monkey, BovinePig (84%), Rabbit (83%), Rhesus Monkey (98%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CP27 antibody CTX antibody CYP27 antibody CYP27A1 antibody sterol 26-hydroxylase mitochondrial antibody 5-beta-cholestane-3-alpha 7-alpha 12-alpha-triol 26-hydroxylase antibody sterol 27-hydroxylase antibody vitamin D(3) 25-hydroxylase antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) |
| Cellular Localization | Mitochondrion membrane |
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using CYP27A1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CYP27A1 antibody detects CYP27A1 protein by western blot analysis. A. 50 µg rat liver lysate/extract 7.5% SDS-PAGE CYP27A1 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse Liver 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CYP27A1 antibody detects CYP27A1 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. CYP27A1 antibody diluted at 1:500.| Positive Control | A549 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
