Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CYP2C8 antibody detects CYP2C8 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. CYP2C8 antibody dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed HeLa, using CYP2C8 antibody at 1:500 dilution.| Product Name | CYP2C8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CYP2C8 (cytochrome P450, family 2, subfamily C, polypeptide 8)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP2C8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CPC8 antibody CYPIIC8 antibody MP-12/MP-20 antibody CYP2C8 antibody cytochrome P450 IIC2 antibody cytochrome P450 subfamily IIC (mephenytoin 4-hydroxylase) polypeptide 8 antibody flavoprotein-linked monooxygenase antibody s-mephenytoin 4-hydroxyla |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (5) |
| Cellular Localization | Endoplasmic reticulum membrane , Microsome membrane |
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CYP2C8 antibody detects CYP2C8 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. CYP2C8 antibody dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using CYP2C8 antibody at 1:500 dilution.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
