Application
WB analysis of extracts from various cell lines using CYP51A1 antibody.Application
ICC/IF analysis of MCF-7 cells using CYP51A1 antibody (green) and DAPI (blue).| Product Name | CYP51A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CYP51A1This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Immunogen | Recombinant protein of human CYP51A1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LDM CP51 cytochrome P450 family 51 subfamily A polypeptide 1 P450L1 CYPL1 CYP51 P450-14DM |
|---|---|
| Target | CYP51A1 |
| Molecular Weight(MW) | 56 kDa (note) |
Application
WB analysis of extracts from various cell lines using CYP51A1 antibody.
Application
ICC/IF analysis of MCF-7 cells using CYP51A1 antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
