| Product Name | Cystatin A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [WR - 23 / 2 / 3 / 3] to Cystatin ACystatins are endogenous inhibitors of lysosomal proteases that form a superfamily that is classified into three families (families 1,2 and 3) according to the homology of the amino acid sequences. Cystatin A (also termed acid cysteine proteinase inhibitor, ACPI, stefin A, and epidermal SH- proteinase inhibitor) belongs to family 1 of the cystatin superfamily of proteins. These proteins are natural inhibitors of cysteine proteinases like cathepsin B, H, L, and S. Cystatin A, a 98 amino acid protein (11-13 kDa), is detected in normal squamous epithelium, follicular dendritic cells in lymphoid tissues, thymic epithelial cells (in Hassalls corpuscles and in medulla), liver cells, granulocytes and basal epithelial cells in the prostate. Cystatin A is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of cystatin A and tumor progression, in several malignancies. Also, Cystatin A has been implicated in aging of tissues, Alzheimer disease, viral infections and inflammatory processes. Antibodies that react specifically with cystatin A are useful tools for studying structure-function relationships and cellular or subcellular localization of the molecule. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | WR-23/2/3/3 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC-P: Use at a dilution of 1/50 - 1/100. Pretreatment with trypsin is required for paraffin sections. IHC-Fr: Use at a dilution of 1/50 - 1/100. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 184600 CSTA 1475 STF1 Cystatin A P01040 STFA |
|---|---|
| Target | Cystatin A |
| Cellular Localization | Cytoplasmic |
| Positive Control | Brain |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/50 - 1/100. Pretreatment with trypsin is required for paraffin sections. IHC-Fr: Use at a dilution of 1/50 - 1/100. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: Sodium Azide; Constituents: PBS, BSA |
