| Product Name | Cystatin A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [WR - 23 / 2 / 3 / 3] to Cystatin ACystatins are endogenous inhibitors of lysosomal proteases that form a superfamily that is classified into three families (families 1,2 and 3) according to the homology of the amino acid sequences. Cystatin A (also termed acid cysteine proteinase inhibitor, ACPI, stefin A, and epidermal SH- proteinase inhibitor) belongs to family 1 of the cystatin superfamily of proteins. These proteins are natural inhibitors of cysteine proteinases like cathepsin B, H, L, and S. Cystatin A, a 98 amino acid protein (11-13 kDa), is detected in normal squamous epithelium, follicular dendritic cells in lymphoid tissues, thymic epithelial cells (in Hassalls corpuscles and in medulla), liver cells, granulocytes and basal epithelial cells in the prostate. Cystatin A is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of cystatin A and tumor progression, in several malignancies. Also, Cystatin A has been implicated in aging of tissues, Alzheimer disease, viral infections and inflammatory processes. Antibodies that react specifically with cystatin A are useful tools for studying structure-function relationships and cellular or subcellular localization of the molecule. |
| Immunogen | Full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | WR-23/2/3/3 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| IHC-P: Use at a dilution of 1/4000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at an assay dependent dilution. Detects a band of approximately 11-13 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 184600 CSTA 1475 STF1 Cystatin A P01040 STFA |
|---|---|
| Target | Cystatin A |
| Cellular Localization | Cytoplasmic |
| Positive Control | Human Thymus tissue |
|---|---|
| Application Notes | IHC-P: Use at a dilution of 1/4000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at an assay dependent dilution. Detects a band of approximately 11-13 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide |
