Application
Detection of cytochrome c in human cells. Cytochrome c migrates as a 13 kDa species Lane 1: cytoplasmic fraction Lane 2: nuclear fractionApplication
Detection of cytochrome c in human cells. Lane 1: cytoplasmic fraction, Lane 2: nuclear fraction. Cytochrome c migrates as a 13 kDa species.| Product Name | Cytochrome C antibody [SJL2-4(apo)] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [ SJL2 - 4( apo )] to Cytochrome CCytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis. |
| Immunogen | Recombinant full length protein (Horse). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | SJL2-4(apo) |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 12 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseMonkeyRatGoatSheep |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CYC, 123970, 54205, CYCS, Cytochrome C, P99999, HCS, THC4 |
|---|---|
| Target | Cytochrome C |
| Cellular Localization | Cytoplasmic and Mitochondrial |
Application
Detection of cytochrome c in human cells. Cytochrome c migrates as a 13 kDa species Lane 1: cytoplasmic fraction Lane 2: nuclear fraction
Application
Detection of cytochrome c in human cells. Lane 1: cytoplasmic fraction, Lane 2: nuclear fraction. Cytochrome c migrates as a 13 kDa species.| Positive Control | 293T cells |
|---|---|
| Application Notes | WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 12 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
