Application
ICC/IF analysis of H-4-II-E cells with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.Application
IHC-P analysis of rat liver tissue with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min.Application
IHC-P analysis of rat kidney tissue with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min.Application
ICC/IF analysis of PC12 cells with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:100 (green). Nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.Application
ICC/IF analysis of HeLa cells with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.| Product Name | Cytochrome P450 2C11 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytochrome P450 2C11The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). EET’s, which exhibit vasodilation activity, are formed when an epoxide group is inserted between the unsaturated carbons of AA in positions 5,6; 8,9; 11,12; 14,15. EET’s are produced in cerebral cortical tissue, coronary arteries and vascular endothelium. EET’s are converted from AA by the 2C11 family of P450’s whose expression is induced by testosterone and is therefore not generally found in females. |
| Immunogen | Synthetic peptides corresponding to residues D(49) I G Q S I K K F S K V(60) and Q(491) R A D S L S S H L(500) of rat Cytochrome P450 2C11. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-FIHC-PWB |
| IHC-P: 1:100 - 1:500: |
|
| Species Reactivity | HumanRatCat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cyp2c11 CYP2CII Cyp2c cytochrome P450 subfamily 2 polypeptide 11 |
|---|---|
| Target | Cytochrome P450 2C11 |
Application
ICC/IF analysis of H-4-II-E cells with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.
Application
IHC-P analysis of rat liver tissue with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min.
Application
IHC-P analysis of rat kidney tissue with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min.
Application
ICC/IF analysis of PC12 cells with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:100 (green). Nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.
Application
ICC/IF analysis of HeLa cells with (right) or without (left) Cytochrome P450 2C11 antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.| Application Notes | IHC-P: 1:100 - 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% sodium azide. |
