Application
Cytokeratin 13 antibody detects Cytokeratin 13 protein at cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Cytokeratin 13 antibody dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 13 antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 13 antibody at 1:200 dilution.Application
Cytokeratin 13 antibody detects KRT13 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 10% SDS-PAGE Cytokeratin 13 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Cytokeratin 13 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 13 (keratin 13)The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 13. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWBIHC-W |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseZebra Fish |
| Predicted Reactivity | RatRat (80%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK13 antibody K13 antibody MGC161462 antibody MGC3781 antibody KRT13 antibody cytokeratin 13 antibody keratin-13 antibody keratin type I cytoskeletal 13 antibody CK-13 antibody cytokeratin-13 antibody keratin 13 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) |
Application
Cytokeratin 13 antibody detects Cytokeratin 13 protein at cytosol on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Cytokeratin 13 antibody dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 13 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 13 antibody at 1:200 dilution.
Application
Cytokeratin 13 antibody detects KRT13 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract 10% SDS-PAGE Cytokeratin 13 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
