Application
IHC Image using - Detection of Cytokeratin 15 by IHC in Human TonsilApplication
Formalin fixed paraffin embedded human skin stained with Cytokeratin 15 antibody .| Product Name | Cytokeratin 15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LKH15] to Cytokeratin 15Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is down regulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is down regulated to maintain the activated phenotype. |
| Immunogen | A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | LKH15 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 is required prior to immunostaining. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | K1CO 3866 K15 P19012 KRT15 148030 Cytokeratin 15 Cytokeratin15 CK15 |
|---|---|
| Target | Cytokeratin 15 |
| Tissue Specificity | Cytokeratin 15 is a type I cytokeratin without a defined type II partner. Cytokeratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. |
| Light Chain Type | kappa |
Application
IHC Image using - Detection of Cytokeratin 15 by IHC in Human Tonsil
Application
Formalin fixed paraffin embedded human skin stained with Cytokeratin 15 antibody .| Application Notes | This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 is required prior to immunostaining. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.09% Sodium Azide |
