Application
Sample(30 ug whole cell lysate) A:HeLa S3 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using Cytokeratin 20 antibody at 1:500 dilution.| Product Name | Cytokeratin 20 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cytokeratin 20 (keratin 20)The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cytokeratin 20. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (87%), Rat (80%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CK-20 antibody CK20 antibody K20 antibody KRT21 antibody MGC35423 antibody KRT20 antibody cytokeratin-20 antibody keratin-20 antibody cytokeratin 20 antibody protein IT antibody keratin type I cytoskeletal 20 antibody keratin 20 anti |
|---|---|
| Target | Cytokeratin 20 |
| Molecular Weight(MW) | 48 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample(30 ug whole cell lysate) A:HeLa S3 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using Cytokeratin 20 antibody at 1:500 dilution.| Positive Control | HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
