Application
Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein at cytoplasm on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Cytokeratin 20 antibody [N2C2], Internal diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20 antibody at 1:100 dilution.| Product Name | Cytokeratin 20 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cytokeratin 20 (keratin 20)The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 20. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CK-20 antibody CK20 antibody K20 antibody KRT21 antibody MGC35423 antibody KRT20 antibody cytokeratin-20 antibody keratin-20 antibody cytokeratin 20 antibody protein IT antibody keratin type I cytoskeletal 20 antibody keratin 20 anti |
|---|---|
| Target | Cytokeratin 20 |
| Molecular Weight(MW) | 48 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Cytokeratin 20 antibody [N2C2], Internal detects Cytokeratin 20 protein at cytoplasm on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Cytokeratin 20 antibody [N2C2], Internal diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20 antibody at 1:100 dilution.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
