Application
ICC/IF analysis of A549 cells using DAPI (blue) and Cytokeratin 20 antibody [AT10D8] at a dilution of 1:200 (green).Application
WB analysis of (1) recombinant human Cytokeratin 20 (25ng), (2) HeLa and (3) A431 lysates (40ug of lysates) using Cytokeratin 20 antibody [AT10D8] at a dilution of 1:3000.Application
FACS analysis of A549 cells using an isotype control (black) or Cytokeratin 20 antibody [AT10D8] at 2-5ug for 1,000,000 cells (red).| Product Name | Cytokeratin 20 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [AT10D8] to Cytokeratin 20The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human KRT20 (1-424aa) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AT10D8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| WB: 1:3000. FACS: 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | K20 antibody KRT20 antibody keratin 20 antibody KRT21 antibody CK-20 antibody CK20 |
|---|---|
| Target | Cytokeratin 20 |
| Light Chain Type | kappa |
Application
ICC/IF analysis of A549 cells using DAPI (blue) and Cytokeratin 20 antibody [AT10D8] at a dilution of 1:200 (green).
Application
WB analysis of (1) recombinant human Cytokeratin 20 (25ng), (2) HeLa and (3) A431 lysates (40ug of lysates) using Cytokeratin 20 antibody [AT10D8] at a dilution of 1:3000.
Application
FACS analysis of A549 cells using an isotype control (black) or Cytokeratin 20 antibody [AT10D8] at 2-5ug for 1,000,000 cells (red).| Application Notes | WB: 1:3000. FACS: 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -23C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
