Application
IHC Image using - Detection of Cytokeratin 20 by IHC in Human Colon Carcinoma| Product Name | Cytokeratin 20 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Cytokeratin 20Cytokeratin 20 is less acidic than the other type I cytokeratins and is of particular interest because of its restricted range of expression. This antibody reacts with cytokeratin polypeptide of 46 kD in immunoblott analyses. Studies have shown that a marked difference exists in the expression of cytokeratin 20 among various carcinomas. |
| Immunogen | Cytokeratins isolated from human micro dissected villi of human duodenal mucosa. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | KS 20.8 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic digestion of formalin fixed paraffin tissue sections is required prior to immunostaining. Can also be used for: Western Blotting (1:50 to 1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 54474 CK20 K20 KRT20 P35900 Cytokeratin20 KRT21 Cytokeratin 20 MGC35423 608218 |
|---|---|
| Target | Cytokeratin 20 |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
IHC Image using - Detection of Cytokeratin 20 by IHC in Human Colon Carcinoma| Positive Control | Skin |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic digestion of formalin fixed paraffin tissue sections is required prior to immunostaining. Can also be used for: Western Blotting (1:50 to 1:100): |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
