| Product Name | Cytokeratin 20 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal to Cytokeratin 20Monoclonal Mouse Anti-Human Cytokeratin 20 |
| Immunogen | Cytokeratin preparation of villi of human duodenal mucosa. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | Ks20.8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-F |
| Used to identify and differentiate different carcinomas. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. For optimal results, tissue sections should either be treated with high-temperature based antigenic unmasking technique, or with proteolytic enzymes such as AutoZyme or Pronase. The optimal conditions should be determined by the individual laboratory. This antibody is not suitable for use on cryostat sections or cell smears due to non-specific cross-reaction with negative epithelia. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 54474 CK20 K20 KRT20 P35900 Cytokeratin20 KRT21 Cytokeratin 20 MGC35423 608218 |
|---|---|
| Target | Cytokeratin 20 |
| Tissue Specificity | This antibody reacts with the 46 kD cytokeratin polypeptide 20, a type I cytokeratin that is found in duodenal epithelium, glandular gastric and pyloric epithelium, urothelium and Merkel cells of the epidermis. It does not cross-react with other cytokeratin polypeptides or other intermediate filaments. This cytokeratin is expressed in colon carcinoma, and to a lesser degree in gastric carcinoma. Other positive carcinomas include gall bladder, bile duct, ductal cells of pancreas, mucinous ovarian tumors and transitional cell carcinomas. Carcinoma of breast, lung, endometrium and non-mucinous tumors of ovary are not recognized byThis antibody. Merkel cell carcinomas of the skin show strong reactivity withThis antibody. |
| Positive Control | Human Normal Colon, Colon Carcinoma |
|---|---|
| Application Notes | Used to identify and differentiate different carcinomas. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. For optimal results, tissue sections should either be treated with high-temperature based antigenic unmasking technique, or with proteolytic enzymes such as AutoZyme or Pronase. The optimal conditions should be determined by the individual laboratory. This antibody is not suitable for use on cryostat sections or cell smears due to non-specific cross-reaction with negative epithelia. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 1X PBS buffer with 0.1% sodium azide. |
