Application
Immunohistochemistry staining of human prostate (paraffin section) using anti-cytokeratin 5+18Application
Western Blot analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1: MW standard Lane 2: immunostaining with anti-cytokeratin 5+18 Lane 3: immunostaining with Isotype mouse IgG1 control| Product Name | Cytokeratin 5 + 18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [C-50] to Cytokeratin 5 + 18The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq] |
| Immunogen | Tissue / cell preparation (Human). Cytoskeleton preparation from HeLa cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | C-50 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseDogPigRatHamsterSheepBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Cytokeratin 5+18 3852 KRT18 CK5 K18 CYK18 P13647 K5 EBS2 P05783 KRT5 KRT5A 131800 Cytokeratin5+18 3875 148070 DDD |
|---|---|
| Target | Cytokeratin 5 + 18 |
| Tissue Specificity | This antibody recognises the 58 kDa Cytokeratin 5 and the 45 kDa Cytokeratin 18 |
Application
Immunohistochemistry staining of human prostate (paraffin section) using anti-cytokeratin 5+18
Application
Western Blot analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1: MW standard Lane 2: immunostaining with anti-cytokeratin 5+18 Lane 3: immunostaining with Isotype mouse IgG1 control| Application Notes | ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.09% sodium azide |
