| Product Name | Cytokeratin 5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [XM26] to Cytokeratin 5Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex. |
| Immunogen | Prokaryotic recombinant protein corresponding to 103 amino acid portion of the C-terminal region of the cytokeratin 5 molecule (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | XM26 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| IHC-P: 1/50 - 1/75. An incubation period of 30 minutes at room temperature is recommended. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | 3852 Cytokeratin 5 CK5 P13647 K5 EBS2 Cytokeratin5 KRT5 KRT5A 131800 |
|---|---|
| Target | Cytokeratin 5 |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | Skin |
|---|---|
| Application Notes | IHC-P: 1/50 - 1/75. An incubation period of 30 minutes at room temperature is recommended. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: Sodium Azide. Constituents: Tissue culture supernatant |
